KMID : 0613820170270020180
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Journal of Life Science 2017 Volume.27 No. 2 p.180 ~ p.186
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Total Phenols, Flavonoid Contents, and Antioxidant Activity of Spirodela polyrhiza Extracts
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Song Won-Young
Choi Jeong-Hwa
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Abstract
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We investigated the antioxidant activities of water and ethanol extracts from Spirodela polyrhiza (SP) through in vitro assays. The total phenolic contents of SP water and ethanol extracts were 52.75-293.4 and 60.12-398.4 mg/g, respectively. The total flavonoid content of SP ethanol extract (38.25-159.4 mg/g) was higher than that of SP water extract (38.25-67.75 mg/g). The water and ethanol extracts from SP scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2'-azino-di-2-ethyl-benzothia-zoline sulfonate (ABTS) radical in a dose-dependent manner in the concentration range of 100?2,500 ¥ìg/ml. The DPPH radical scavenging activity of the SP ethanol extract (2.87%-59.5%) was higher than that of the water extract (4.12%-81.52%). The IC50s of the DPPH radical scavenging activity of water and ethanol extracts were 2,100 and 1,034 ¥ìg/ml respectively. The ABTS radical scavenging activities of SP water and ethanol extracts were 8.30%-83.16% and 13.11%-8.34% respectively. The IC50s of the ABTS radical scavenging activity of SP water and ethanol extracts were 798.7 and 457.1 ¥ìg/ml, respectively. The reducing power activities of SP water and ethanol extracts were 0.055-1.122 and 0.140-1.428, respectively (500-4,000 ¥ìg/ml). The soybean lipoxygenase (SLO) radical scavenging activities of SP water and ethanol extracts were 157.7%-168.0% and 148.0%-169.4%, respectively. These results suggest that the water and ethanol extracts of SP may be useful as a potential antioxidant.
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KEYWORD
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Antioxidant, DPPH radical, flavonoid, phenol, Spirodela polyrhiza
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